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HPLC-ECD System

HTEC-600

N E W

★   ★   ★

Ultra-High Sensitivity and Stability

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​超高感度 / 高安定

★ 感度はドーパミン・セロトニンで30fg 以下
★ 革新的セルの設計・無脈動ポンプのシステム設計
★ 冷却器とヒーターによる温度制御システム
★ ECDの最適化されたアプリケーション

 

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all in One

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一体型で省スペース

送液ポンプ、デガッサ、恒温槽、電気化学検出器を一体化。
これにより流速が500μL/min を超える(カラムサイズが大きなもの)
ような他検出器の分析アプリケーションの実施が可能になりました
※ 従来のモデルでは庫内に入れる事が出来なかったサイズの長い
カラムも使用可能。幅広い分析アプリケーションに対応しました

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作用電極の洗浄や交換・メンテナンスが容易
 

コンパクトでシンプルな設計の為、作用電極の交換をするのに工具は不要です。
またメンテナンスが驚くほど容易です

purge

高速送液を行えます。稼働時間を設定できるので
液が枯れる心配はありません。エア抜き、液交換
を自動で行えるので一般的なHPLC のトラブル

で最も多いポンプによるエラーが大幅に改善
されました。

Timer

ポンプ、ECD それぞれを時間制御でON/OFF

する機能です。稼働時間を制限できるので移動

相の消費を最小限に抑える事が可能です。

このTIMER 機能によってポンプやECD を効率

的に稼働させスムーズなサンプル測定へ移行

する事が出来ます。

 

L.Switch

HTEC-600 からELS-500 を制御する事が可能

です。アミノ酸分析や比較的夾雑が出やすい

サンプルの場合クロマトグラム後半に出てくる

夾雑を除去することは分析の効率化につながる

ので、ELS-500を手軽に使用できることは実験

する上で大きなメリットとなります。

 

​ HTEC-600が得意とする物質

★ドーパミン ★DOPAC ★HVA ★3MT ★L-DOPA ★ノルエピネフリン  ★MHPG  ★ノルメタネフリン ★エピネフリン  
★セロトニン ★5-HIAA ★メラトニン  ★フェノール類  ★ アセチルコリン ★コリン ★ヒドロキシラジカル  ★アスコルビン酸
★トコフェロール  ★エストラジオール  ★8OH-dG ★ニトロチロシン ★GSH ( グルタチオン) ★GSSG ( グルタチオン) 
★アスパラギン酸 ★グルタミン酸 ★グルタミン ★グリシン  ★タウニン ★アラニン  ★GABA  など

測定可能な物質はこれだけではありません。お問い合わせ下さい。

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HTEC-600

HPLC-ECD SYSTEM

仕様


<測定原理>***********************************************************************

方式            HPLC-ECD 法

<検出器>*************************************************************************

検出方式          アンペロメトリック

印加電圧        0 ~ ±2,000 mV、1 mV step

作用電極方式      薄層式

作用電極種類      グラファイト( 標準)、ピュアグラファイト、
            グラシーカーボン、白金、金、銀

作用電極ガスケット   TFE 製 厚さ25 μm または50 μm

参照電極        銀/ 塩化銀 (塩化リチウム封入液絡式)

対極 (補助電極)     SUS316

接液部材質       PEEK、SUS316

記録計出力信号     ±10 V アナログ (0.1 nA = 1 mV)

時定数         1.0 sec、1.5 sec、3.0 sec

信号入力        SIGNAL IN: 接点信号300 msec 以上で検知
            M.INJECTOR SIGNAL IN:オープン/ クローズ信号
            切換えから300 sec で検知

外部機器制御信号    0 sec:信号入力検知と同時に接点信号 (1 sec) 出力
            3 sec:信号入力検知から3 秒後に接点信号 (1 sec) 出力
            6 sec:信号入力検知から6 秒後に接点信号 (1 sec) 出力


<送液ポンプ>*********************************************************************

送液方式        直列ダブルプランジャー往復動方式

1 ストローク容量     80 μL

ピストン材質      サファイア

設定流量範囲       1 ~ 3000 μL/min

安定流量範囲      100 ~ 2000 μL/min

接液部材質        PEEK、サファイア、ルビー、PTFE、PCTFE

耐圧           20 MPa

脈流低減機構       自動脈流制御


<脱気装置>***********************************************************************

方式           連続真空膜透過式

流路数          2 流路

内部容量         上段:約300 μL/ 流路

             下段:約7.5 mL/ 流路

<恒温槽>*************************************************************************

方式            ペルチェ素子による加熱/ 冷却空気循環式

設定温度範囲        15 ~ 50℃ (1℃step)

温調精度           ±0.1℃

<全 体>***************************************************************************

寸法           290 W x 380 D x 435 H mm ( 突起部を含まず)

重量           23.2 kg

電源           AC 100 ~ 240 V 50/60 Hz 300 VA

​関連した文献

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2021

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2020

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  Behavior.


-  Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

-  Mice carrying a schizophrenia-associated mutation of the Arhgap10 gene are vulnerable to the effects of methamphetamine treatment on cognitive function:
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2019

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-  Kappa Opioid Receptors Drive a Tonic Aversive Component of Chronic Pain

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2018

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2017

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2016

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-  Brain regions and monoaminergic neurotransmitters that are involved in mouse ambulatory activity promoted by bupropion

2015

-  Injection of corticotropin-releasing hormone into the amygdala aggravates visceral nociception and induces noradrenaline release in rats

2014

-  Pregerminated Brown Rice Enhanced NMDA Receptor/CaMKIIα Signaling in the Frontal Cortex of Mice 
 
-  Monitoring Serotonin: Why Faster is Better

2013

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-  Pain-Related Depression of the Mesolimbic Dopamine System in Rats: Expression, Blockade by Analgesics, and Role of Endogenous κ-opioids

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2011

-  Oral administration of glycine increases extracellular serotonin but not dopamine in the prefrontal cortex of rats

2009

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2007

-  Cortical Acetylcholine Release Is Lateralized during Asymmetrical Slow-Wave Sleep in Northern Fur Seals

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