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ERP-10

Roller pump

AtmosLM Sampling Peristaltic Pump 2 channels, 1 rotor.

Developed for use as a sampling pump with AtmosLM. Stable at low flow rates. Works for push-pull sampling too.​

​journal article

 

AtmosLM™ Microdialysis with Pressure Canceling Technology

In Vivo Large Molecule Sampling

What is AtmosLM™ Pressure Canceling Technology?

Recovering large molecules, such as cytokines or neuropeptides, by microdialysis has always been challenging due

to large pore size membranes that are required. The membranes will often leak because the pressure inside the

microdialysis probe is comparatively high.

What is AtmosLM™ Pressure Canceling Technology

Atmos LM microdialysis probes have a vent hole in upper part of the probe that prevents the pressure inside the

microdialysis membrane rising above normal atmospheric pressure. The new AtmosLM technology enabled to apply

a large pore membrane to microdialysis probes without allowing leakage. However, the situation with normal

microdialysis probes is quite different because the membrane is part of a closed system. A number of factors

associated with the outlet side of the probe often impact the pressure at the membrane and cause leakage or

variation in the recovery rate.

Sampling Pump (ERP-10)

To remove the microdialysis sample from the probe, a low-pulse sampling pump is

required. This is in addition to the standard syringe pump used for infusion. Eicom

has developed a new peristaltic pump, Model ERP-10, specifically for the

AtmosLM system.

・Two channels on one unit.

・Flow rate as low as 0.5 µL.

・Six rollers per pump head.

Is AtmosLM™ the same as “Push-Pull”?

No, but the new AtmosLM pressure canceling technology is sometimes thought of

as an improved, push-pull technique. The AtmosLM requires significantly shorter

equilibration time before inserting probes because there is no requirement that the

flow rates of the inlet and outlet pumps must be exactly matched. Before the

AtmosLM technique, small mismatches in the pumps led to long equilibrations times

and/or a change in the fluid volume in the brain of the animal.

Recovery Rate Test Condition

Membrane Length  4 mm membrane
Flow Rate        1 μL/min for Syringe Pump and Peristaltic Pump

aCSF                            including 0.15% BSA

In Vitro Amyloid ß40 Recovery Rate

Recovery of IL-6 (26 kDa)