ホワイト.png

ENO-30

» Suitable for all biological fluids( 1-50 µL injections)

» Both Nitrite and Nitrate from single measurement
» Sensitivity is better than 0.1 pmol (10 nM for 10
» Easily automated to run 144 samples per day

​Nitrite and Nitrate Analyzer

The ENO-30 continues the tradition of Eicom’s successful
ENO-30 NOx analyzer. The ENO-30 system is a specialized
HPLC for separating and detecting nitrite and nitrate in various
biological fluids. The system makes use of a simple Griess
reaction, in which nitrite reacts to form a light absorbing
compound that can easily be detected. However, compared to a typical plate or tube based Griess assay, the ENO-30 greatly enhances
sensitivity and reproducibility. In addition, high throughput analysis is achieved by pairing the instrument with an HPLC autosampler,
an impossible feat for the gas phase chemiluminescent detectors sometimes used to detect nitric oxide. Data collection, calibration,
and analysis are handled by Eicom’s Envision software package.
Suitable for all biological fluids(1-50 μL injections)

Nitrite and Nitrate Appear as Two Peaks
Each injection measures both nitrite and nitrate.

Peak sizes are proportional to concentration in the sample.

Perfect for Biological Samples
Sensitive enough for small volume biological samples. The typical sample
is just 1-50 μ L of blood plasma, urine, saliva, breathe condensate, tissue
homogenate, cell culture supernatant, or microdialysate. Simple sample
preparation consist of the addition of an equal volume of methanol to
inactive and precipitate proteins, whereas low protein samples can be
direct injected without prior manipulation.

How does it work?

​journal article